Journal: EMBO Reports

Article Title: Role and mechanism of PVN–sympathetic–adipose circuit in depression and insulin resistance induced by chronic stress

doi: 10.15252/embr.202357176

Figure Lengend Snippet: A, B Representative images of immunohistochemical staining of CRH after chronic restraint stress treatment. C Real‐time PCR analysis of CRH in hypothalamus under chronic restraint stress, two‐tailed unpaired t ‐test, t (10) = 1.016, P = 0.3336, control group n = 6, restraint group n = 6. D, E ELISA results showing serum ACTH (D, Mann–Whitney U ‐test, P = 0.0311) and CORT levels (E, two‐tailed unpaired t ‐test, t (14) = 2.178, P = 0.0470) under chronic restraint stress, control group n = 7, restraint group n = 9. F, G Representative images showing mcherry (F, F″) and hM3D‐mcherry (G, G") expression in PVN, immunohistochemical staining of CRH (F′ and G′). H Real‐time PCR analysis of CRH in hypothalamus after chronically reactivating PVN neurons, two‐tailed unpaired t ‐test, t (10) = 0.1353, P = 0.8951, mcherry group n = 6, hM3D group n = 6. I, J ELISA results showing serum ACTH (I, two‐tailed unpaired t ‐test, t (16) = 1.215, P = 0.2420) and CORT levels (J, two‐tailed unpaired t ‐test, t (16) = 1.616, P = 0.1257) after chronically reactivating PVN neurons, mcherry group n = 8, hM3D group n = 10. Data information: Shapiro–Wilk test and F ‐test were used to test the normality and equal variance assumptions, respectively. Two‐tailed t ‐tests were performed to assess differences between two experimental groups with normally distributed data and equal variance. Two‐tailed t ‐tests with Welch's correction were used when a two‐sample comparison of means with unequal variances. For non‐normally distributed data, Mann–Whitney U ‐tests were performed to compare two groups. P < 0.05 was considered statistically significant. * P < 0.05. Data are presented as means ± SEM. Source data are available online for this figure.

Article Snippet: Mouse ACTH ELISA Kit (Elabscience, E‐EL‐M0079c), QuicKey Pro Mouse CORT ELISA Kit (Elabscience, E‐OSEL‐M0001), Mouse Leptin/LEP ELISA Kit (BOSTER Biological Technology, EK0438).

Techniques: Immunohistochemistry, Staining, Real-time Polymerase Chain Reaction, Two Tailed Test, Enzyme-linked Immunosorbent Assay, MANN-WHITNEY, Expressing, Comparison

Journal: Nutrients

Article Title: Evaluation of the Relationship Between Orexin A, Peptide YY, AgRP, and POMC Levels and Sleep Disorders in Children with Malnutrition

doi: 10.3390/nu18030377

Figure Lengend Snippet: Box plots showing the distribution of the orexin A, proopiomelanocortin (POMC), agouti-related protein (AgRP), and peptide yy (PYY) levels in the malnourished children and typically developing (TD) healthy controls. The Mann–Whitney U test was used to compare the peptide levels between the two groups.

Article Snippet: Human POMC, orexin A, AgRP, and PYY enzyme-linked immunosorbent assay (ELISA) kits (Shanghai Korain Biotech Co., Ltd., Shanghai, China) were used to measure the plasma peptide levels.

Techniques: MANN-WHITNEY